供应美国GLAS-COL细胞破碎仪BIONEB?详细介绍
高压细胞破碎系统BIONEB?
●超声波破碎机无法破碎的细胞,可轻易破
●对于许多细胞来说,仅通过1或2次操作就可使破碎率达到90%
●细胞破碎时,对细胞伤害极小
●不产热,反而使样品降温。只需花极少的时间,该装置能在30毫升/分的流量下破碎细胞。
●大量细胞破碎时,可连续使用
BioNeb细胞粉碎装置是印第安纳大学分子细胞生物学教室的Dr. Stefan J. Surzycki,Dr. Robert K. Togasaki,Mr. Masahiko开发;在BioNeb细胞破碎装置中,利用He,N2,Ar等的气压(约10~250psi的范围),一边去热(度)破碎细胞,一边喷雾,用撞击的剪切力短时间,并有效地击溃细胞,用***离析来处理细胞悬浮液(5 ml以上)。不热发生(利用气化热冷却样品);细胞破碎速度:30 ml /分钟,有效率几乎不费时间,对细胞不造成任何外力负担。
BioNeb细胞破碎效果模拟图
How the BioNeb system works?
In the process of droplet formation, large molecules or cells
suspended in the liquid being nebulized are forcefully distributed
from the liquid into the forming secondary droplet. This creates a transient laminar flow in the microcapillary "nebulization" channel formed between the surface of the liquid and the forming secondary droplet. The laminar flow in the capillary channel exerts sufficient shearing forces to break cells. The shearing force created depends on the gas pressure applied, the type of gas, and the viscosity of the liquid. This makes it possible to precisely regulate the magnitude of the force applied during nebulization. Therefore, the same instrument can be used for very gentle opening of the cells as necessary in organelle isolation, or for thorough breakage of the cells when preparation of a cell extract is desired.
BioNeb細胞破碎裝置與其他破碎法比較
| 速度 | 正確性 | 再現性 | 非熱變性 | 方便性 | 清洗性 | 其他效能 |
BioNeb細胞破碎装置 | ★★★ | ★★★ | ★★★ | ★★★ | ★★★ | ★★★ | 高效率,小心打開細胞 |
酵素處理 | - | - | ★ | ★★★ | ★★ | N/A | 高價,無法大量處理,後續純化處理比較麻煩 |
研磨(材) | ★ | - | - | - | ★★★ | ★ | 需在大量處理的情況,價格高,浪費時間,容易產熱 |
Yeda Press | ★★ | - | ★ | ★ | ★ | ★ | 單價高、不能單獨分離胞器、調整條件麻煩 |
French Press | ★★ | - | ★ | ★ | ★ | ★ | 價格貴、不能單獨分離胞器、調整條件麻煩 |
超音波破碎 | ★ | - | - | - | ★ | ★ | 不能單獨分離胞器、無法破碎酵母菌、噪音太大 |
应用范围
细菌
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Gram(-)rods
Escherichiacoli ![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Gram(-)photoautotrophs (cyanobacteria)
Synechocystis-PCC#6803
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Gram(+)rods, spore forming
Bacillus subtilis ![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Gram(+)rods, non spore forming; acid fast (mycobacteria)
Mycobacteriam philei 酵母及丝状菌
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Yeasts (unicellular; monomorphic)
Scaccharomyces albicans
Scaccharomyces K-6
Scaccharomyces OC-2
Candida albicans
Schizosaccharomyces
Bretanomyces bruxerlenis ![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Filamentous fungi
Penicillium chrysogenum
Aspergillus
Fusarium
Rhizopus
Trichophyton 动物细胞
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Protozoa
Eimeria tenella (unsporulateoocysts)
Trypanosoma cruzi (epimastigotes)
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Metazoa: mammalian cell lines CHO, CHL, Nb2, 3T3, L, M1 & M2
植物细胞
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Algae
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Chlamydonas reinhardtii
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Higher plants
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Asparagus officinalis
![*](https://res.gys.cn/img/mobile/common/loading/circle-loading.svg)
Soybean tissue culture
Each system includes a universal positioner and baseplate, flowmeter, 1/8" dia. cut-to-length tubing for reservoir inlet/outlet, 12" length of auxiliary inlet/outlet tubing, 6' length of 5/32" O.D. tubing for gas supply, and connection fittings. (Depending on specific gas cylinders and regulators, some additional fittings may be required.)
Catalog Number | List Price | Description | Product Weight (lbs) |
105A BN3010 | 74916.00 | BioNeb? disruption system with cylinder for 5- to 10-ml sample volume. Includes universal positioner. | - - |
105A BN3015 | 74916.00 | BioNeb? disruption system with cylinder for 10- to 30-ml sample volume. Includes universal positioner. | - - |
105A BN3025 | 76236.00 | BioNeb? disruption system with cylinder for both 5- to 10-ml and 10- to 30-ml sample volumes. Include universal positioner. | - - |
105A BN4000 | 8580.00 | Two-stage gas regulator for Helium or Nitrogen. 3000 psi max. inlet pressure. 0-250 psi outlet pressure. | - - |